Induction and Detection of Mitophagy

• DOI: 10.1007/978-1-0716-2071-7_14
• Publication Date: 2022-01-01T03:55:42Z
• Authors: Maria A. Yapryntseva; Boris Zhivotovsky; Vladimir Gogvadze
• Keywords: Macroautophagy; Autophagy; Mitophagy; Mitochondria

Mitophagy, a process of selective elimination of mitochondria by autophagy, is a mechanism of mitochondrial quality control that maintains mitochondrial network functionality. The elimination of damaged mitochondria through autophagy requires two steps: induction of general autophagy and priming of damaged mitochondria for selective autophagic recognition. Mitophagy impairment is linked to various pathologies; thus, removal of malfunctioning or even harmful mitochondria is vital to cellular physiology. Here, we describe methods that can be applied to the investigation of mitophagy.