Immunoglobulin Gene Mutational Status Assessment by Next Generation Sequencing in Chronic Lymphocytic Leukemia

• DOI: 10.1007/978-1-0716-2115-8_10
• Publication Date: 2022-05-27T06:02:51Z
• Authors: Anne Langlois de ...; Myriam Boudjoghra; Clotilde Bravetti; Marine Armand; Mikaël Salson; Mathieu Giraud; Frederic Davi
• Keywords: Immunoglobulin gene; breakpoint cluster region

Abstract B cell receptor (BcR) immunoglobulins (IG) display a tremendous diversity due to complex DNA rearrangements, the V(D)J recombination, further enhanced by somatic hypermutation process. In chronic lymphocytic leukemia (CLL), mutational load of clonal BcR IG expressed leukemic cells constitutes an important prognostic and predictive biomarker. Here, we provide reliable methodology capable determining status genes in CLL using high-throughput sequencing, starting from or RNA.