One-Step Next-Generation Sequencing of Immunoglobulin and T-Cell Receptor Gene Recombinations for MRD Marker Identification in Acute Lymphoblastic Leukemia

• DOI: 10.1007/978-1-0716-2115-8_3
• Publication Date: 2022-05-27T06:02:51Z
• Authors: Patrick Villarese; Chrystelle Abdo; Matthieu Bertrand; Florian Thonier; Mathieu Giraud; Mikaël Salson; Elizabeth Macintyre
• Keywords: Recombination, Genetic; 0303 health sciences; One step; Neoplasm, Residual; B cell receptor; High-Throughput Nucleotide Sequencing; Immunoglobulins; [SDV.CAN]Life Sciences [q-bio]/Cancer; [SDV.MHEP.HEM]Life Sciences [q-bio]/Human health and pathology/Hematology; Precursor Cell Lymphoblastic Leukemia-Lymphoma; 3. Good health; Genes, T-Cell Receptor; 03 medical and health sciences; [SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology; Next-generation sequencing; Humans; T cell receptor; [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM]

AbstractWithin the EuroClonality-NGS group, immune repertoire analysis for target identification in lymphoid malignancies was initially developed using two-stage amplicon approaches, essentially as a progressive modification of preceding methods developed for Sanger sequencing. This approach has, however, limitations with respect to sample handling, adaptation to automation, and risk of contamination by amplicon products. We therefore developed one-step PCR amplicon methods with individual barcoding for batched analysis for IGH, IGK, TRD, TRG, and TRB rearrangements, followed by Vidjil-based data analysis.