Isolation and Identification of Dendritic Cell Subsets from Human and Mouse Tumors

• DOI: 10.1007/978-1-0716-2938-3_14
• Publication Date: 2023-03-11T14:03:07Z
• Authors: Yamila Rocca; Aurélien Voissière; Jenny Valladeau-G...; Nathalie Bendriss...
• Keywords: Mice; Phagocytosis; Neoplasms; Humans; Animals; Dendritic Cells; Lymphocyte Activation

Dendritic cells (DCs) are professional antigen-presenting cells (APCs) that have the ability to orchestrate adaptive and innate immune responses by antigen phagocytosis and T cell activation across different inflammatory settings such as tumor development. As specific DC identity and how these cells interact with their neighbors is still not fully understood, it remains a challenge to unravel DC heterogeneity, particularly in human cancers. In this chapter, we describe a protocol to isolate and characterize tumor-infiltrating DCs.