In Vivo and In Vitro Assay to Address Dendritic Cell Antigen Cross-Presenting Capacity

• DOI: 10.1007/978-1-0716-2938-3_17
• Publication Date: 2023-03-11T14:03:07Z
• Authors: Pengju Ou; Lifen Wen; Hai Ni; Cliff Y. Yang
• Keywords: Cross-Presentation; CTL*

Antigen cross-presentation by dendritic cells is an important pathway to prime CD8+ T cells in infections, cancer, and other immune-mediated pathologies. Particularly in cancer, cross-presentation of tumor-associated antigens is crucial for an effective antitumor CTL response. The mostly accepted cross-presentation assay is to use chicken ovalbumin (OVA) as a model antigen and then utilize OVA-specific TCR transgenic CD8+ T (OT-I) cells to measure the cross-presenting capacity. Here we describe in vivo and in vitro assays to measure the function of antigen cross-presentation using cell-associated OVA.