Measuring Circadian Oscillations of Cytosolic-Free Calcium in Arabidopsis thaliana

• DOI: 10.1007/978-1-4939-0700-7_15
• Publication Date: 2014-05-02T10:35:34Z
• Authors: Timothy J. Hearn; Alex A. R. Webb
• Keywords: Aequorin; Photoprotein

Circadian oscillations of cytosolic-free calcium can be measured in plants by observing luminescence of the bioluminescent calcium binding protein aequorin. Here we describe the use of intensified photon-counting CCD cameras to measure circadian oscillations in aequorin bioluminescence from Arabidopsis thaliana.