Single-Cell Genome and Transcriptome Processing Prior to High-Throughput Sequencing

• DOI: 10.1007/978-1-4939-2519-3_5
• Publication Date: 2015-06-04T05:34:06Z
• Authors: Ana M. Aransay; Laura Barcena; Aintzane Gonzalez...; Nuria Macias-Camara
• Keywords: Single cell sequencing; Cancer genome sequencing

Single-cell genome and transcriptome characterizations will probe to be decisive within the stem cells research, especially to describe appropriately the genetic impact of the diverse stem cells populations that are present in each organism. In the present chapter, we describe in detail how to prepare sequencing libraries out of single cells, for whole genome DNA and mRNA sequencing.