Methods for TALEN Evaluation, Use, and Mutation Detection in the Mosquito Aedes aegypti

• DOI: 10.1007/978-1-4939-2932-0_13
• Publication Date: 2015-10-06T14:14:18Z
• Authors: Sanjay Basu; Azadeh Aryan; Mary Etna Haac; Kevin M. Myles; Zach N. Adelman
• Keywords: Gene drive

The generation and study of transgenic Aedes aegypti mosquitoes provides an essential tool for elucidating the complex molecular biology of this important vector. Within the field, genetic manipulation has surpassed the proof of principle stage and is now utilized in both applied and theoretical vector control strategies. The application of new instruments, technologies and techniques allows ever more controlled experiments to be conducted. In this text we describe microinjection of Ae. aegypti embryos in the context of evaluating and performing genomic editing with transcription activator-like effector nucleases (TALENs).