Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line
0301 basic medicine
0303 health sciences
03 medical and health sciences
A549 Cells
Cell Survival
Humans
Epithelial Cells
Transfection
Lipids
Lung
Cell Proliferation
Plasmids
DOI:
10.1007/978-1-4939-3652-6_13
Publication Date:
2016-06-14T08:40:21Z
AUTHORS (2)
ABSTRACT
Trials of transfection in eukaryotic cells are essential tools for the study of gene and protein function. They have been used in a wide range of research fields. In this chapter, a method of transient transfection of the A549 cell line, human lung cells of alveolar epithelium, with an expression plasmid is described. In addition, the fundamental characteristics of this experimental procedure are addressed.
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