Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line

• DOI: 10.1007/978-1-4939-3652-6_14
• Publication Date: 2016-06-14T08:40:21Z
• Authors: Elena Marcos-Vadillo; Asunción García-S...
• Keywords: A549 Cells; Genes, Reporter; Humans; Cloning, Molecular; Promoter Regions, Genetic; Transfection; Polymorphism, Single Nucleotide; Luciferases, Renilla; 3. Good health

The development of reporters systems has simplified the study of promoter activity in different areas of knowledge, and represents an easy and fast approach to study genetic variations. In this chapter, we show a transfection protocol of A549 lung epithelial cells with a reporter vector, using the Luciferase-Renilla dual system for studying the variations caused by several polymorphisms in the promoter region of a gene.