Effects of HDACi on Immunological Functions

• DOI: 10.1007/978-1-4939-6527-4_7
• Publication Date: 2016-10-19T09:26:03Z
• Authors: René Winkler; Christian Kosan
• Keywords: Lipopolysaccharides; B-Lymphocytes; Indoles; Cell Survival; Immunomagnetic Separation; Cell Cycle; Carbazoles; Immunoglobulins; Cell Differentiation; Flow Cytometry; Hydroxamic Acids; Immunoglobulin Class Switching; Histone Deacetylases; 3. Good health; Histone Deacetylase Inhibitors; Mice, Inbred C57BL; Mice; Benzamides; Animals; Cytokines; Cell Proliferation

Histone deacetylase inhibitors (HDACi) are used as therapeutics for several B cell-derived malignancies. Furthermore, they have been shown to modulate the response of the immune system, like the B cell function. HDACi treatment affects differentiation, proliferation, and survival of B cells. Here we describe how to investigate the effects of HDACi treatment on naïve B cells regarding class-switch recombination (CSR) in vitro using flow cytometry.