Telomere Strand-Specific Length Analysis by Fluorescent In Situ Hybridization (Q-CO-FISH)

• DOI: 10.1007/978-1-4939-6892-3_4
• Publication Date: 2017-03-21T05:31:30Z
• Authors: Isabelle Ourliac-...; Arturo Londoño-Va...
• Keywords: [SDV] Life Sciences [q-bio]; DNA Replication; [SDV.BIO]Life Sciences [q-bio]/Biotechnology; [SDV]Life Sciences [q-bio]; [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology; Humans; [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology; Telomere; Chromosomes; Cells, Cultured; In Situ Hybridization, Fluorescence; [SDV.BIO] Life Sciences [q-bio]/Biotechnology

The implementation of quantitative approaches in telomere chromosome-oriented FISH (telomeric CO-FISH) allows the assessment of the relative efficiency of lagging versus leading strand telomere replication and thus provides information on the implicated mechanisms. Here, we describe a simple method for telomere strand-specific analyses and discuss its potential applications.