Selection of High-Producing Clones Using FACS for CHO Cell Line Development

• DOI: 10.1007/978-1-4939-6972-2_9
• Publication Date: 2017-05-10T03:56:53Z
• Authors: Clair Gallagher; Paul S. Kelly
• Keywords: Cell Sorting; Biopharmaceutical; clone (Java method)

Cell line development aims to generate and select clones with desirable characteristics. One of the most important parameters for biopharmaceutical cell selection is cell-specific productivity (Qp) or the quantity of product produced per cell per day. Fluorescence-activated cell sorting (FACS) is a powerful, high-throughput technique that facilitates multiparametric characterization and isolation of individual cell clones from heterogeneous populations. Here, we describe a FACS-based method for section of high-producing CHO cell clones.