Preparation of Rat Hippocampal Organotypic Cultures and Application to Study Amyloid β-Peptide Toxicity

Rats, Sprague-Dawley Amyloid beta-Peptides Organ Culture Techniques Animals, Newborn brain slices; ex vivo; hippocampus; neuropharmacology; organotypic culture; rat; toxicity; beta-amyloid; molecular biology; genetics Animals Rats, Wistar Hippocampus Rats 3. Good health
DOI: 10.1007/978-1-4939-7571-6_24 Publication Date: 2017-12-08T10:59:42Z
ABSTRACT
Hippocampal organotypic cultures constitute a very easy but delicate method widely used to study amyloid β-peptide toxicity. This ex vivo technique is performed on tissues isolated from newborn rats. Here, we describe a protocol for the preparation and culture of hippocampal organotypic slices that can be maintained for 14-21 days and their application to the study of amyloid β-peptide toxicity.
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