Preparation of Rat Hippocampal Organotypic Cultures and Application to Study Amyloid β-Peptide Toxicity

• DOI: 10.1007/978-1-4939-7571-6_24
• Publication Date: 2017-12-08T10:59:42Z
• Authors: Maria Rosanna Bro...; Roberta Facchinetti; Caterina Scuderi
• Keywords: Rats, Sprague-Dawley; Amyloid beta-Peptides; Organ Culture Techniques; Animals, Newborn; brain slices; ex vivo; hippocampus; neuropharmacology; organotypic culture; rat; toxicity; beta-amyloid; molecular biology; genetics; Animals; Rats, Wistar; Hippocampus; Rats; 3. Good health

Hippocampal organotypic cultures constitute a very easy but delicate method widely used to study amyloid β-peptide toxicity. This ex vivo technique is performed on tissues isolated from newborn rats. Here, we describe a protocol for the preparation and culture of hippocampal organotypic slices that can be maintained for 14-21 days and their application to the study of amyloid β-peptide toxicity.