Dynamic Light Scattering Analysis to Dissect Intermediates of SNARE-Mediated Membrane Fusion

• DOI: 10.1007/978-1-4939-8760-3_4
• Publication Date: 2018-10-13T08:34:54Z
• Authors: Byoungjae Kong; Yoosoo Yang; Dae-Hyuk Kweon
• Keywords: Proteolipids; Lipid Bilayers; Liposomes; SNARE Proteins; Membrane Fusion; Dynamic Light Scattering; Recombinant Proteins; Software

Dynamic light scattering (DLS) spectroscopy provides rapid information on the size distribution of a large number of particles in a mixture. Vesicle sizes change during the merger of lipid bilayers, and DLS analysis can provide rapid, accurate, and non-perturbative quantification of the size distribution of proteoliposomes in SNARE-dependent membrane fusion. In this chapter, we describe the methodologies and reagents used for DLS spectroscopy in a biochemical and biophysical study of SNARE-mediated membrane fusion.