Using the Dunn Chemotaxis Chamber to Analyze Primary Cell Migration in Real Time

0301 basic medicine 0303 health sciences Chemotaxis Macrophages Cell Culture Techniques 610 Cell Separation Time-Lapse Imaging 3. Good health 03 medical and health sciences Mice 616 Animals Cell Migration Assays
DOI: 10.1007/978-1-61779-207-6_4 Publication Date: 2011-07-11T21:48:57Z
ABSTRACT
The directed migration of cells (chemotaxis) occurs not only during wound healing and inflammatory responses but also during embryonic development. However, the intracellular signaling pathways that enable a cell to detect a chemoattractant and subsequently migrate toward the source are not clearly defined. The Dunn chemotaxis chamber in conjunction with time-lapse microscopy is a powerful tool that enables the user to observe directly the morphological response of cells to a chemoattractant in real time. Here, using the Dunn chemotaxis chamber, we describe the response of murine bone marrow-derived macrophages to colony stimulating factor-1. This is a particularly useful protocol as it can be adapted to study bone marrow-derived macrophages isolated from genetically modified mice and thus study the requirement of a specific protein in cell migration and chemotaxis.
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