Electrophoretic Implementation of the Solution-Depletion Method for Measuring Protein Adsorption, Adsorption Kinetics, and Adsorption Competition Among Multiple Proteins in Solution

• DOI: 10.1007/978-1-62703-462-3_12
• Publication Date: 2013-08-05T17:54:10Z
• Authors: Hyeran Noh; Naris Barnthip; Purnendu Parhi; Erwin A. Vogler
• Keywords: Protein Adsorption

The venerable solution-depletion method is perhaps the most unambiguous method of measuring solute adsorption from solution to solid particles, requiring neither complex instrumentation nor associated interpretive theory. We describe herein an SDS-gel electrophoresis implementation of the solution--depletion method for measuring protein adsorption and protein-adsorption kinetics. Silanized-glass particles with different surface chemistry/energy and hydrophobic sepharose-based chromatographic media are used as example adsorbents. Electrophoretic separation enables quantification of adsorption competition among multiple proteins in solution for the same adsorbent surface, demonstrated herein by adsorption--competition kinetics from binary solution.