Intrastriatal transplantation of mouse bone marrow-derived stem cells improves motor behavior in a mouse model of Parkinson’s disease

• DOI: 10.1007/978-3-211-73574-9_16
• Publication Date: 2007-12-27T18:43:42Z
• Authors: D. Offen; Y. Barhum; Y.-S. Levy; A. Burshtein; H. Panet; T. Cherlow; E. Melamed
• Keywords: Male; Neurons; Cell Survival; Dopamine; Blotting, Western; Green Fluorescent Proteins; Cell Differentiation; Mice, Transgenic; Motor Activity; Mesenchymal Stem Cell Transplantation; Corpus Striatum; Mice, Inbred C57BL; Amphetamine; Mice; Microscopy, Fluorescence; Parkinsonian Disorders; Cell Movement; Image Processing, Computer-Assisted; Animals; Bone Marrow Transplantation

Strategies of cell therapy for the treatment of Parkinson's disease (PD) are focused on replacing damaged neurons with cells to restore or improve function that is impaired due to cell population damage. In our studies, we used mesenchymal stromal cells (MSCs) from mouse bone marrow. Following our novel neuronal differentiation method, we found that the basic cellular phenotype changed to cells with neural morphology that express specific markers including those characteristic for dopaminergic neurons, such as tyrosine hydroxylase (TH). Intrastriatal transplantation of the differentiated MSCs in 6-hydroxydopamine-lesioned mice led to marked reduction in the amphetamine-induced rotations. Immunohistological analysis of the mice brains four months post transplantation, demonstrated that most of the transplanted cells survived in the striatum and expressed TH. Some of the TH positive cells migrated toward the substantia nigra. In conclusion, transplantation of bone marrow derived stem cells differentiated to dopaminergic-like cells, successfully improved behavior in an animal model of PD suggesting an accessible source of cells that may be used for autotransplantation in patient with PD.