Free Fatty Acid Liberation and Cellular Swelling During Cerebral Ischemia: the Role of Excitatory Amino Acids

• DOI: 10.1007/978-3-7091-9334-1_64
• Publication Date: 2012-07-28T20:56:44Z
• Authors: Yoichi Katayama; T. Kawamata; T. Maeda; T. Tsubokawa
• Keywords: Microdialysis

We previously demonstrated the occurrence of cellular swelling during cerebral ischemia in vivo using microdialysis. 14C-Sucrose was pre-perfused into extracellular space (ECS) as an ECS marker, and detected increase concentration 14C-sucrose. In present study, we examined rats time courses FFA relation to swelling, role excitatory amino acids (EAAs) these events. A pair microdialysis probes were placed hippocampus bilaterally. One probe perfused with modified Ringer solution containing kynurenic acid (KYN, 10 mM), a broad-spectrum EAA antagonist, other control. At 30 minutes after initiation perfusion, induced by decapitation. The brain subjected microwave irradiation at 0-8 decapitation, levels dorsal measured high performance liquid chromatography. course determined separate group animals described method. It found that arachidonic stearic began demonstrate rapid period from 1 2 following induction. oleic palmitic similar but less marked increase. rate became 4 minutes, suggesting transient superimposed on background slow roughly corresponded timing early swelling.(ABSTRACT TRUNCATED AT 250 WORDS)