Fermented Asterina pectinifera with Cordyceps militaris Mycelia Induced Apoptosis in B16F10 Melanoma Cells

• DOI: 10.1007/978-94-024-1079-2_91
• Publication Date: 2017-08-28T04:54:12Z
• Authors: Yon-Suk Kim; Eun-Kyung Kim; Jin-Woo Hwang; Jin-Soo Kim; Hakju Kim; Xin Dong; Sithranga Boopath...; Sang-Ho Moon; Byong-Tae Jeon; Pyo-Jam Park
• Keywords: Mice; Mycelium; Cell Survival; Cell Line, Tumor; Asterina; Cordyceps; Fermentation; Melanoma, Experimental; Animals; Antineoplastic Agents; Apoptosis; 3. Good health

This prime objective of this study was to explore the anti-cancer activity of fermented Asterina pectinifera with Cordyceps militaris mycelia (FACM) in B16F10 murine melanoma cells. The effect of FACM on cell viability was assessed using MTT assay. Furthermore, the effect of FACM was compared with unfermented A. pectinifera on cell viability. The results demonstrated that the fermented FACM extract has a higher inhibitory activity on the proliferation of B16F10 murine melanoma cells than unfermented A. pectinifera. In addition, FACM also promoted the expression of pro-apoptotic protein Bax leading to stimulate apoptosis in B16F10 cells. Therefore the present study demonstrates that the FACM might be a potential effective anti-cancer agent, as a result of its stronger anti-proliferative effect and apoptosis inducing effect than A. pectinifera or C. militaris on melanoma cells.