Structure and expression of a light-inducible shoot-specific rice gene

0303 health sciences Chloroplasts Base Sequence Light Ribulose-Bisphosphate Carboxylase Molecular Sequence Data Oryza DNA Genes, Plant Open Reading Frames 03 medical and health sciences Gene Expression Regulation Sequence Homology, Nucleic Acid Consensus Sequence Amino Acid Sequence Protein Processing, Post-Translational Plant Proteins
DOI: 10.1007/bf00019157 Publication Date: 2004-10-30T07:53:01Z
ABSTRACT
By differential screening of a cDNA library of two-week-old rice seedlings cDNA clones were obtained, corresponding to shoot-specific mRNAs. By sequence analysis two of these clones were found to be rbcS cDNA clones. The mRNA corresponding to a third cDNA clone (COS5) displayed an expression pattern similar to the expression pattern of rbcS genes. The mRNA (800 bases) was light-inducible and encoded by a single-copy gene. The genomic clone (GOS5) was isolated and the intron/exon structure was determined by comparing the nucleotide sequences of the mRNA and the genomic clone. The gene contains two introns. Transcription start sites were determined by S1-nuclease mapping and primer extension. The start site obtained by both methods is located 87 bp upstream of the translation start site and 23 bp downstream of TATA box-like sequence. In the 5' non-coding region motifs can be found that are homologous to sequences in promoters that are light- or UV-inducible or confer leaf-specific expression. The open reading frame present in GOS5 codes for a protein (15 kDa) that contains a putative chloroplast transit peptide and does not show any significant homology to protein sequences in the NBRF protein database.
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