Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from structurally unrelated single carbon sources by newly isolated Pseudomonas sp. EL-2

• DOI: 10.1007/bf00128596
• Publication Date: 2004-11-02T18:23:37Z
• Authors: Hongjoo Son; Sangjoon Lee
• Keywords: 0301 basic medicine; 03 medical and health sciences

A Pseudomonas sp. EL-2 strain capable of synthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] was isolated from activated sludge. For simulation of P(3HB-co-3HV) production in the cells, deficiency of nutrients such as NH4 +, SO4 2- and Mg2+ was crucial and the maximum content of P(3HB-co-3HV) could reach 46% on NH4 +-deficient medium. This organism synthesized P(3HB-co-3HV) with 3HV monomer in the range from 1.9 to 49.3 mol% from unrelated single carbon sources such as glucose, fructose, propionate, or sorbitol. P(3HB-co-3HV)s containing a higher fraction of 3HV were produced by adding propionic acid to glucose medium.