High frequency transformation of Alcaligenes eutrophus producing poly-?-hydroxybutyric acid by electroporation

• DOI: 10.1007/bf00152996
• Publication Date: 2004-11-06T01:47:28Z
• Authors: Hae-Chul Park; Kyung-Jun Lim; Jin-Seo Park; Yong-Hyun Lee; Tae-Lin Huh
• Keywords: Kanamycin; Alcaligenes; Strain (injury); Plasmid preparation

A strain of Alcaligenes eutrophus producing poly-β-hydroxybutyric acid was successfully transformed by the electroporation. The plasmid used was a broad host range plasmid pKT230 conferring kanamycin resistance. The optimum yield of transformant was 0.8×102/μg DNA when 50 μl competent cells at 1010/ml were pulsed by 11.5 kV/cm for 5 ms with 1 μg DNA. Plasmid DNA in the A. eutrophus transformant was stably maintained as a monomeric structure.