Induction and production of cellulases by l-sorbose in Trichoderma reesei

• DOI: 10.1007/bf00250321
• Publication Date: 2004-09-16T10:36:20Z
• Authors: Mikio Kawamori; Yasushi Morikawa; Seigo Takasawa
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences

Cellulase production in Trichoderma reesei mutants was induced by l-sorbose, known to be an inhibitor of β-1,3-glucan synthesis. In the experiments the washed mycelia were used as resting cells. For CMCase induction over 24 h using T. reesei PC-3-7, the most effective pH, temperature and l-sorbose concentration were 2.8, 28° C and 0.3 mg/ml, respectively. Comparison with other cellulase inducers showed that the inductive level of CMCase by l-sorbose was similar to that by sophorose, known to be the most potent inducer of cellulases. Since the induction of CMCase was inhibited completely by 10 μg of cycloheximide per ml, the induction process was considered to involve de novo synthesis. Although l-sorbose had the effective inducibility of CMCase, the assimilation rate of l-sorbose was very low in T. reesei PC-3-7.