In vivo/in vitro labeling of red blood cells with 99mTc

• DOI: 10.1007/bf00253769
• Publication Date: 2004-09-16T06:03:10Z
• Authors: R. Bauer; I. Haluszczynski; H. Langhammer; W. Bachmann
• Keywords: Kinetics; 03 medical and health sciences; Erythrocytes; 0302 clinical medicine; Tin; Heart Function Tests; Humans; Technetium; In Vitro Techniques; Gastrointestinal Hemorrhage; Radionuclide Imaging; 3. Good health

Red blood cells (RBC) were labeled with 99mTc in vitro after in vivo pretreatment with a stannous solution. The whole in vivo/in vitro procedure took 30-40 min. Pretinning was carried out by injection of either 6 mg DTPA and 0.6 mg Sn++ (Sn-DTPA) or 5 mg pyrophosphate and 0.7 mg Sn++ (Sn-PYP). The results of 1,356 patients were evaluated. The labeling yield was 89.7% (mean) after pretinning with Sn-DTPA and 88.2% following Sn-PYP pretreatment, the median values being 94% and 92%, respectively. The new method was successfully used in over 2,000 patients who had a radionuclide-ventriculography and in 38 patients studied for localization of occult gastrointestinal bleeding. Adverse side effects have never been observed.