Agrobacterium-mediated transformation of sunflower (Helianthus annuus L.) shoot apices: transformation patterns

• DOI: 10.1007/bf00437911
• Publication Date: 2004-11-12T01:38:59Z
• Authors: Monique Burrus; Jean Molinier; Christophe Himber; Reiner Hunold; Roberte Bronner; Philippe Rousselin; G�nther Hahne
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences

Apical segments of embryonic axes of sunflower (Helianthus annuus L.) embryos were submitted to co-culture experiments with a disarmed strain of Agrobacterium tumefaciens, harbouring a plasmid coding for the marker enzyme β-glucuronidase. The expression patterns of this marker were analysed at different developmental stages of the regenerated shoots. The results are consistent with the hypothesis that transformed shoots originate from transformation events that have occurred within the existing meristems. Two of the resulting chimaeric plants have been analysed in detail, and some representative gene integration patterns are presented.