Proposal for a procedure for complete inactivation of the Creutzfeldt-Jakob disease agent

• DOI: 10.1007/bf01310679
• Publication Date: 2005-03-26T00:23:16Z
• Authors: F. Taguchi; Y. Tamai; K. Uchida; R. Kitajima; H. Kojima; T. Kawaguchi; Y. Ohtani; S. Miura
• Keywords: Medical microbiology; Creutzfeldt-Jakob Syndrome

We have examined complete inactivation conditions on brain homogenates from mice affected with Creutzfeldt-Jakob disease agent, and recommend for routine use a reliable procedure first treating the affected materials with 1 N NaOH for 60 min and then autoclaving at 121 degrees C for 30 min.