A tissue culture method for the detection of bacterial enterotoxins

• DOI: 10.1007/bf01666023
• Publication Date: 2005-05-13T21:26:52Z
• Authors: D. R. Maneval; R. R. Colwell; S. W. Joseph; R. Grays; S. T. Donta
• Keywords: Microbiological culture

Specific standard steps are described for the implementation of the Y1 mouse adrenal tumor cell line in evaluating the production of bacterial enterotoxins and cytotoxins. The importance of standardization of methods for Y1 cell culture, preparation of test materials, and interpretation of results is emphasized. Rationale for utilization of the Y1 cell assay and difficulties of application are discussed. Adaptations for research purposes and potential applications are described.