cAMP-activated chloride channel in the basolateral membrane of the thick ascending limb of the mouse kidney

• DOI: 10.1007/bf01870076
• Publication Date: 2005-07-01T16:00:02Z
• Authors: Marc Paulais; Jacques Teulon
• Keywords: Male; 0303 health sciences; Colforsin; Electric Conductivity; [SDV.BBM.BP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics; Membrane Proteins; In Vitro Techniques; Basement Membrane; Membrane Potentials; [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics; Mice; 03 medical and health sciences; Kidney Tubules; Chlorides; Chloride Channels; 1-Methyl-3-isobutylxanthine; Cyclic AMP; Loop of Henle; Animals

The properties of an anion-selective channel observed in basolateral membranes of microdissected, collagenase-treated, cortical thick ascending limbs of Henle's loop from mouse kidney were investigated using patch-clamp single-channel recording techniques. In basal conditions, single Cl- currents were detected in 8% of cell-attached and excised, inside-out, membrane patches whereas they were observed in 24% of cell-attached and 67% of inside-out membrane patches when tubular fragments were preincubated with Forskolin (10(-5) M) or 8-bromo-cAMP (10(-4) M) and isobutylmethylxanthine (10(-5) M). The channel exhibited a linear current-voltage relationship with conductances of about 40 pS in both cell-attached and cell-free membrane configurations. A PNa+/PCl- ratio of 0.05 was estimated in the presence of a 142/42 mM NaCl concentration gradient applied to inside-out membrane patches. Anionic selectivity of the channel followed the sequence Cl- greater than Br- greater than NO3- much greater than F-; gluconate was not a permeant species. The open-state probability of the channel increased with membrane depolarization in cell-attached, i.e., in situ membrane patches. In excised, inside-out, membrane patches, the channel was predominantly open with the open-state probability close to 0.8 over the whole range of potentials tested (-60 to +60 mV). The channel activity was not a function of internal calcium concentration between 10(-9) and 10(-3) M. We suggest that this Cl- channel, whose properties are distinct from those in other epithelia, could account for the well-documented conductance which mediates Cl- exit in the basolateral step of NaCl absorption in thick ascending limb of Henle's loop.