A refocused and optimized HNCA: Increased sensitivity and resolution in large macromolecules

• DOI: 10.1007/bf01875530
• Publication Date: 2005-07-02T18:14:07Z
• Authors: B. T. Farmer; R. A. Venters; L. D. Spicer; M. G. Wittekind; L. Müller
• Keywords: Isoenzymes; Carbon Isotopes; 0303 health sciences; 03 medical and health sciences; Magnetic Resonance Spectroscopy; Nitrogen Isotopes; Protein Conformation; Humans; Mathematics; Carbonic Anhydrases

A 3D optimized, refocused HNCA experiment is described. It is demonstrated to yield a dramatic increase in sensitivity when applied to [13C, 15N]-labeled human carbonic anhydrase II, a 29-kDa protein. The reasons for the gain in sensitivity are discussed, and 3 distinct areas for further development are indicated.