Germ line transmission and expression of alacZ containing transgene in tilapia (Oreochromis niloticus)

• DOI: 10.1007/bf01969426
• Publication Date: 2005-07-30T12:45:52Z
• Authors: Samsul Alam; Andrew Popplewell; Norman Maclean
• Keywords: 0303 health sciences; 03 medical and health sciences

Transgenic tilapia were produced using a carp β-actinlacZ gene construct and the transmission of the transgene to F1 progeny was followed. Of 36 founder fish analysed, 9 (25%) were found to be transgenic in at least one tissue and two of these were found to transmit the transgene to progeny. One male produced only three out of 28 transgene DNA positive progeny (10.7%) but none of these were found to express the transgene. The other male produced 243 out of 1601 (15.2%)lacZ expressing embryos; 40 of these were analysed by Southern blotting and all were found to be positive for the presence of the transgene but revealed two different patterns of transgene arrangement. Three different expression patterns were also observed and presumed to be a result of multiple integration events and resulting position effects.