The role of siderophores in potato tuber yield increase by Pseudomonas putida in a short rotation of potato

• DOI: 10.1007/bf01977588
• Publication Date: 2005-08-02T06:11:09Z
• Authors: P. A. H. M. Bakker; J. G. Lamers; A. W. Bakker; J. D. Marugg; P. J. Weisbeek; B. Schippers
• Keywords: Pseudomonas putida; Crop Rotation

The effect of treatment of potato seed tubers with Pseudomonas putida isolate WCS358 on tuber yield was studied in different crop rotations at the Experimental Farm 'De Schreef', near Lelystad. With untreated plants, tuber yield in a 1:3 (short) rotation compared to yield in a 1:6 (long) rotation of potato was decreased by 11% at 86 days (seed tuber harvest) and by 14~ at 130 days (ware potato harvest) after seeding. Seed tuber treatment with the wild-type isolate WCS358 increased tuber yield with 13070 in a short rotation of potato 86 days after seeding, whereas a siderophore-negative Tn5 transposon mutant of this isolate had no effect on tuber yield. Seed tuber treatment with the wild-type isolate or the siderophore-negative mutant in a long rotation of potato had no effect on tuber yield. At 130 days after seeding no effect of any of the seed tuber treatments was found in both short and long rotations of potato. Root colonization by siderophore-producing Tn5 transposon mutants of WCS358 was decreased at the end of the growing season. No difference in root colonization between siderophore-producing and siderophore-negative Tn5 transposon mutants was found at 130 days after seeding. Siderophore production seems to be a prerequisite in potato tuber yield increase by WCS358 under field conditions. This is the first time that the involvement of siderophores in growth stimulation has been demonstrated in the field.