Trocar site recurrence is unlikely to result from aerosolization of tumor cells

• DOI: 10.1007/bf02053799
• Publication Date: 2005-08-13T21:58:20Z
• Authors: R. L. Whelan; G. J. Sellers; J. D. Allendorf; D. Laird; M. D. Bessler; R. Nowygrod; M. R. Treat
• Keywords: Aerosolization; Surgical oncology; Colorectal Surgery

This study was undertaken to investigate the ability of a high-pressure CO2 environment aerosolize tumor cells in both vitro and vivo models. (An aerosol is defined as stable gaseous suspension insoluble particles). Also, this designed determine if rapid desufflation capable transporting fluid laden with cells.The four experiments were performed an 18.9-1 plastic vessel fitted two 7-mm ports compliant latex balloon affixed top. After insufflation, desufflated through sterile soluset containing 25 ml culture media that subsequently emptied into dish, incubated for weeks, periodically assessed growth. At bottom vessel, one following placed: Study 1 2, B16 melanoma or colon 26 liquid media; 3, saline solution; 4, several pieces solid tumor. In Studies cell preparations subjected conditions (pneumo): 1) static pneumo 15 30 mmHg (10 minute dwell); 2) continuous flow (CF) (1O l) while maintaining pressure vessel. only CF tested. Between 6 12 determinations each condition preparation. consisted Spraque Dawley rats received intraperitoneal injections 10-5 0.1 media. Two laparoscopic placed abdomen, insufflation desufflation. groups were: 1, mmHg; 2 at 5 10 mmHg. Desufflation via same collecting device handled identical manner described above. The experiment transport fluid-containing cells; model used. To prevent complete loss volume on desufflation, wire coil inside balloon. Twenty 20 x 10(-6) insufflated l gas. There three differed degree which agitated before follows: no agitation; moderate agitation coat lower walls coil; 3) maximum entire verify viability cells, end study, sample peritoneal washing These samples served positive controls.In studies following. weeks incubation, growth noted any 124 test dishes. 14 control all demonstrated Zero 18 experimental dishes grew All Groups growth, whereas five six did so Group 3 (maximally desufflation). Again, controls cells.We unable demonstrate formation performed. experiment, desufflation-related but when surface coated desufflation.Aerosols are not likely form. Free most found suspension. potential means cell-laden fluid.