Gel mobility shift scanning of the acetate-inducible promoters fromNeurospora crassa reveals a common co-inducible DNA-binding protein

• DOI: 10.1007/bf02174030
• Publication Date: 2005-09-20T23:54:24Z
• Authors: T. Mizote; M. Bibbins; P. J. Sheffield; I. F. Connerton
• Keywords: 0303 health sciences; Binding Sites; Base Sequence; Neurospora crassa; Molecular Sequence Data; Sequence Analysis, DNA; Acetates; DNA-Binding Proteins; Fungal Proteins; 03 medical and health sciences; Gene Expression Regulation, Fungal; DNA, Fungal; Promoter Regions, Genetic

The promoter regions of four acetate-inducible genes of Neurospora crassa, acu-3, acu-5, acu-8 and acu-9, have been sequenced. Using a scanning gel mobility shift assay particular DNA regions in each promoter have been shown specifically to bind partially purified protein extracted from acetate-induced mycelia. The protein-binding regions so defined have common sequence motifs, elements of which are similar to those required for acetate induction in aspergillus nidulans.