Experimental evidence does not support use of the “no-touch” isolation technique in colorectal cancer

• DOI: 10.1007/bf02235045
• Publication Date: 2005-10-21T06:30:29Z
• Authors: Damián García-Olmo; Jesús Ontañón; Dolores C. García...; Mario Vallejo; Jesús Cifuentes
• Keywords: Carcinoembryonic antigen; Surgical oncology; Colorectal Surgery; Circulating tumor cell

The benefits of the "no-touch" isolation technique usually performed to prevent circulation tumor cells are not evident. aim this study was determine whether conventional surgical procedures for treatment colon cancer could provoke detected by a genetic technology.Sixteen patients undergoing resection colorectal and two with irresectable tumors were studied. No patient showed liver or lung metastasis. With specific primers carcinoembryonic antigen, we used reverse transcriptase-polymerase chain reaction analyze biopsy specimens blood samples obtained from antecubital vein before after surgery main drainage when had been extracted. Peritoneal fluid also collected in irrecsectable cases.Amplification cDNA antigen-specific achieved all biopsies peritoneal fluid. In antigen products one them surgery. Only (Dukes C) tumor. serial dilution experiments determined that limit detection method ten 2 ml blood.Our data suggest use no-touch techniques is justified, based on lack evidence indicating detachment at