Determination of L-tryptophan-serum albumin binding by high-performance liquid chromatography

• DOI: 10.1007/bf02344792
• Publication Date: 2006-03-16T00:18:51Z
• Authors: S. F. Sun; Florence Wong
• Keywords: 01 natural sciences; 0104 chemical sciences

Two high-performance liquid chromatography (HPLC) techniques were developed for the determination of binding constants in the interaction of serum albumin with L-tryptophan: internal calibration and external calibration. The results obtained were compared with those obtained by the classical method of equilibrium dialysis and by gel filtration. While all the methods are equally reliable, the internal and external calibration techniques seem to be superior in their simplicity, speed and convenience.