Genotypic identification of staphylococcal enterotoxins and toxic shock syndrome toxin 1 by the enzymatic detection of polymerase chain reaction-amplified DNA

• DOI: 10.1007/bf02350847
• Publication Date: 2006-03-16T06:34:27Z
• Authors: Akio Kimura; Hideo Igarashi; Kimiko Ubukata; Satoru Nakagami; Akio Yamane; Masatoshi Konno
• Keywords: 0301 basic medicine; 03 medical and health sciences; 3. Good health

The enzymatic detection of a polymerase chain reaction product (ED-PCR), a new detection method of PCR-amplified DNA, was evaluated for the identification of staphylococcal enterotoxin (SE) and toxic shock syndrome toxin 1 (TSST-1) genes. A total of 61 Staphylococcus aureus strains, including reference strains and strains isolated from clinical specimens and food poisoning outbreaks, were examined by ED-PCR and by reverse passive latex agglutination (RPLA) phenotypic identification. There was 100% agreement between the genotypic and phenotypic identification of SEA, SEB, SEC, SEE strains and TSST-1. In the case of SED, however, 4 strains were positive by ED-PCR and negative by RPLA. ED-PCR offers an accurate alternative to traditional immunoassays or conventional PCR using electrophoresis for the detection of SE and TSST-1 production yielding results that are more precise than with older techniques.