A direct fluorimetric DNA assay on cell suspensions

• DOI: 10.1007/bf02388262
• Publication Date: 2006-04-10T18:40:03Z
• Authors: M. P. Bonis; N. Germain; J. Frey

An improved direct fluorimetric assay is described using bisbenzimidazol fluorescence at 356 nm excitation and 458 nm emission wavelengths. The turbidity of the medium was shown to have no effect on fluorescence under an absorbance of 0.2 at the excitation wavelength. The method was evaluated by comparisons with a colorimetric DNA assay and cell counting. The linearity of fluorescence was determined up to 15μg/ml of DNA. The method was very reproducible and sensitive to detect 10 ng/ml of DNA and may be used for cell suspensions containing around 5000 cells/ml or more.