Sterol synthesis and viability oferg11 (cytochrome P450 lanosterol demethylase) mutations inSaccharomyces cerevisiae andCandida albicans

• DOI: 10.1007/bf02537115
• Publication Date: 2006-11-29T18:20:00Z
• Authors: M. Bard; N. D. Lees; T. Turi; D. Craft; L. Cofrin; R. Barbuch; C. Koegel; J. C. Loper
• Keywords: Lanosterol; Demethylase

Abstract The identification of the precise structural features yeast sterol molecules required for essential “sparking” function has been a controversial area research. Recent cloning and gene disruption studies in Saccharomyces cerevisiae have shown that C‐24 methylation ( ERG6 ), C‐5 desaturation ERG3 ) Δ 8 ‐Δ 7 isomerization ERG2 are not required, while C‐14 demethylation ERG11 reduction ERG24 each aerobic viability. Earlier observations had indicated demethylase deficient strains could be restored to growth by suppressor mutations caused deficiency desaturase. These were reported synthesize some ergosterol, indicating they contained leaky both , thereby making it imposssible determine whether removal methyl group was availability genes used this study construct contain null mutants . Results show these double viable spontaneously arising suppressors erg3 mutants. erg11 S. compared similar Candida albicans absence lesion.