Chemical characterization and physicochemical behavior of biosurfactants

• DOI: 10.1007/bf02661805
• Publication Date: 2007-07-16T22:43:39Z
• Authors: J. L Parra; J. Guinea; M. A. Manresa; M. Robert; M. E. Mercadé; F. Comelles; M. P. Bosch
• Keywords: Decanoic acid; Chemical structure; Fast atom bombardment; Rhamnolipid

Abstract Microbes have been isolated from soil and water samples after an enrichment culture process with kerosene tested for biosurfactant production by measuring the surface interfacial tensions emulsification power of broths. The isolation characterization extracellular surface‐active agents broth Pseudomonas aeruginosa 44T1 strain made. Preliminary structure identification specific TLC reagents CHCl 3 MeOH (2:1) extracts showed two spots a glycolipidic Rf values 0.70 0.45, respectively, using solvent system :MeOH:H 2 O (65:25:4). Separation active Chromatographic absorption column in Florisil or silica gel further spectroscopic study (IR, 1H‐NMR, 13C‐NMR) chemical degradation techniques (acid alkaline hydrolysis) gave β[β(2‐0‐α‐L‐rhamnopyranosyloxy)decanoyl] decanoic acid β[β (2‐0‐α‐L‐rhamnopyranosyl‐α‐L‐rhamnopyranosyloxy) decanoyloxy] (glycolipid A glycolipid B, respectively) glycolipids detected. new method mass spectrometry, fast atom bombardment spectrometry (FABMS), was used to probe molecular structure. spectra obtained contain weight recognition sequence information signals, they are agreement proposed structures. Physicochemical evaluations were minimum tension 25 mN/m solutions. At pH 7 CMC value 11 ppm. In both cases, at displaced lower 9 it higher concentration values. Glycolipid B (0.2 mN/m) than (1.0 mN/m). pH, whereas had acidic pH.