Optimal conditions for the production of exopolysaccharide by marine microorganismHahella chejuensis

• DOI: 10.1007/bf02936591
• Publication Date: 2008-08-14T15:59:23Z
• Authors: Sung-Hwan Ko; Hyun Sang Lee; Shin Hye Park; Hong Kum Lee
• Keywords: 0303 health sciences; 03 medical and health sciences; 6. Clean water

A marine microorganism, strain 96CJ10356 produced exopolysaccharide, designated as EPS-R. To optimize culture conditions for the production of EPS-R, carbon and nitrogen sources, mineral salts, temperature, and pH were examined. From this study, STN medium for the production of EPS-R was suggested as follows; sucrose 20 g, tryptone 10 g, NaCl 10 g, MgSO4 5 g, CaCl2 1 g, KH2PO4 76 mg, K2HPO4 83 mg, FeCl2 5 mg, MnCl2 1 mg, NaMoO4 1 mg, and ZnCl2 1 mg per liter at pH 7.0. About 9.23 g/L of EPS-R was obtained from STN medium after cultivation for 120 h at 25°C in a 5-liter jar fermentor with an aeration rate of 0.17 vvm. Apparent viscosity and flocculation activity of the culture broth were increased with the production of EPS-R and the maximal values were 415 cP and 1400 unit/mL against 0.5% activated carbon, respectively.