Scoparone from artemisia capillaris inhibits the release of inflammatory mediators in RAW 264.7 cells upon stimulation cells by interferon-γ plus LPS

• DOI: 10.1007/bf02977716
• Publication Date: 2008-11-03T17:05:45Z
• Authors: Seon II Jang; Young-Jun Kim; Woo-Yiel Lee; Kyung Chell Kwak; Seung Hwa Baek; Gyu Beum Kwak; Young-Gab Yun; Tae-Oh Kwon; Hun Taeg Chung; Kyu-Yun Chai
• Keywords: Lipopolysaccharides; 0301 basic medicine; Cell Survival; Plant Extracts; Macrophages; Blotting, Western; Anti-Inflammatory Agents; Nitric Oxide Synthase Type II; Nitric Oxide; Dinoprostone; Cell Line; 3. Good health; Interferon-gamma; Mice; 03 medical and health sciences; Artemisia; Coumarins; Cyclooxygenase 2; Prostaglandin-Endoperoxide Synthases; Animals; Cytokines; Inflammation Mediators; Nitric Oxide Synthase

Scoparone is a major component of the shoot of Artemisia capillaris (Compositae), which has been used for the treatment of hepatitis and biliary tract infection in oriental countries. In the present study we observed that, scorparone exhibited no cytotoxic effect in unstimulated macrophages, but reduced the release of nitric oxide (NO) and prostaglandin E2 (PGE2) upon stimulation by IFN-gamma/LPS or LPS. The inhibitory effects were found to be in conjuction with the suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in IFN-gamma/LPS stimulated RAW 264.7 cells. Moreover, scoparone also attenuated the production of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta and IL-6 in LPS-stimulated RAW264.7 cells. These results suggest that scoparone decreases the production of the inflammatory mediators such as NO and PGE2 in macrophages by inhibiting iNOS and COX-2 expression.