Determination of eperisone in human plasma by liquid chromatography-ESI-tandem mass spectrometry

• DOI: 10.1007/bf02980255
• Publication Date: 2008-10-30T11:53:02Z
• Authors: Min Kyo Jeoung; Eun Sook Jeong; Nam Hee Kim; Chang-Soo Kim; Youn-Bok Chung; Yong-Moon Lee; Su-Youn Ahn; Hwang-Eui Cho; Yong Hwa Lee; Jin Tae Hong; Dong-Cheul Moon
• Keywords: Adult; Male; Propiophenones; Spectrometry, Mass, Electrospray Ionization; Area Under Curve; Calibration; Humans; 01 natural sciences; Chromatography, High Pressure Liquid; 0104 chemical sciences

A sensitive and selective method for the determination of 4'-ethyl-3-methyl-3-piperidinopropiophenone hydrochloride (eperisone hydrochloride) in human plasma was developed and validated. The procedure employed an internal standard and a solvent extraction step followed by chromatography on a Xterra C18 minibore column. Detection was by electrospray ionization tandem mass spectrometry with multiple reaction monitoring. The mass transitions of eperisone and tolperisone (IS) were m/z 260 --> 98 and m/z 246 --> 98, respectively. The method has a limit of detection of 0.1 pg/mL for eperisone based on the three times signal-to-noise value with a linear range from 0.01 to 10.0 ng/mL for the analyte. Extraction recovery was on average 98.6 +/- 7.2% (SD) for eperisone. The Intra- and inter-day assay accuracy ranged from 93 to 114% and precision (RSD) was better than 8.5%. The method was successfully employed to analyze plasma samples and evaluate pharmacokinetics of eperisone in healthy male volunteers.