Characterization of a Novel Hemoglobin-Glutathione Adduct That Is Elevated in Diabetic Patients

• DOI: 10.1007/bf03401868
• Publication Date: 2018-09-03T02:44:41Z
• Authors: Yousef Al-Abed; Sonya VanPatten; Hongwei Li; John A. Lawson; Garret A. FitzGerald; Kirk R. Manogue; Richard Bucala
• Keywords: Dithiothreitol; BETA (programming language); Molecular mass

Typically, a diagnosis of diabetes mellitus is based on elevated circulating blood glucose levels. In an attempt to discover additional markers for the disease and predictors prognosis, we undertook characterization HbA1d3 in diabetic normal patients.PolyCAT A cation exchange chromatography liquid chromatography-mass spectroscopy was utilized separate alpha- beta-globin chains characterize their presence patients.We report as glutathionylated, minor hemoglobin subfraction that occurs higher levels patients (2.26 +/- 0.29%) than individuals (1.21 0.14%, p < 0.001). The alpha-chain spectrum displayed molecular ion m/z 15126 Da, which consistent with predicted native mass HbA0 alpha-globin chain. By contrast, beta-chain showed excess 307 Da (m/z = 16173 Da) versus chain 15866 Da). weight modified regenerated by treatment dithiothreitol, glutathionylated adduct.We propose (HbSSG) forms normally vivo, may provide useful marker oxidative stress potentially other pathologic situations.