Changes in MiRNA-5196 Expression as a Potential Biomarker of Anti-TNF-α Therapy in Rheumatoid Arthritis and Ankylosing Spondylitis Patients

• DOI: 10.1007/s00005-018-0513-y
• Publication Date: 2018-05-09T06:19:36Z
• Authors: Marzena Ciechomska; Krzysztof Bonek; Michal Merdas; Patryk Zarecki; Jerzy Swierkot; Piotr Gluszko; Katarzyna Bogunia...; Wlodzimierz Masli...
• Keywords: Adult; Male; 0303 health sciences; Tumor Necrosis Factor-alpha; Antibodies, Monoclonal; Middle Aged; Real-Time Polymerase Chain Reaction; 3. Good health; Arthritis, Rheumatoid; MicroRNAs; 03 medical and health sciences; C-Reactive Protein; Gene Expression Regulation; Antirheumatic Agents; Humans; Original Article; Female; Spondylitis, Ankylosing; Biomarkers; Aged

In this study, we analysed the expression level of sera circulating miRNA-5196 in rheumatoid arthritis (RA) and ankylosing spondylitis (AS) patients before and after tumor necrosis factor (TNF)-α therapy as biomarkers predicting positive treatment outcome. We enrolled 10 RA patients, 13 AS patients, and 12 healthy individuals in the study. The expression of miRNA-5196 was measured by real-time polymerase chain reaction before and after anti-TNF-α therapy. Disease activity of RA patients was assessed using disease activity score 28 (DAS28), whereas ankylosing spondylitis DAS (ASDAS) was used in AS patients. MiRNA-5196 expression was significantly higher in patients with RA and AS before TNF-α therapy than in those following anti-TNF-α therapy and healthy controls. Changes in miRNA-5196 expression positively correlated with delta DAS28 or delta ASDAS, respectively, following TNF-α therapy. In contrast, changes in C-reactive protein (CRP) levels in RA and AS patients did not positively correlate with DAS28 or ASDAS changes. Receiver-operating characteristic analysis showed better diagnostic accuracy of miRNA-5196 expression both in RA (area under curve (AUC) = 0.87, p = 0.055) and AS patients (AUC = 0.90, p = 0.050) compared to CRP levels in RA (AUC = 0.75, p = 0.201) and AS patients (AUC = 0.85, p = 0.086) upon biologic therapy treatment. Finding novel biomarkers, including miRNA-5196 which allow to predict and monitor anti-TNF-α response, would be of clinical value especially during the early phase of RA or AS development.