Oestrogen-modulated gene expression in the human endometrium

• DOI: 10.1007/s00018-004-4435-y
• Publication Date: 2005-01-21T17:33:44Z
• Authors: C. Punyadeera; H. Dassen; J. Klomp; G. Dunselman; R. Kamps; F. Dijcks; A. Ederveen; A. de Goeij; P. Groothuis
• Keywords: Adult; female reproductive-tract; steroid hormones; 572; extracellular matrix; progesterone; des; Polymerase Chain Reaction; angiogenesis; Endometrium; 03 medical and health sciences; 0302 clinical medicine; Cell differentiation; Humans; implantation; remodelling; breast-cancer; Gelsolin; Menstrual Cycle; Steroid hormones; Glycoproteins; Estradiol; pathway; Gene Expression Profiling; Membrane Proteins; wnt signaling; matrix metalloproteinase-26; Middle Aged; Microarray Analysis; cell differentiation; messenger-rna; Gene Expression Regulation; oocyte donation; physiology; Extracellular matrix remodelling; diethylstilbestrol; Female; Angiogenesis; frizzled related protein

To identify key regulatory mechanisms in the growth and development of the human endometrium, microarray analysis was performed on uncultured human endometrium collected during menstruation (M) and the late-proliferative (LATE-P)-phase of the menstrual cycle, as well as after 24 h incubation in the presence of oestradiol (17beta-E2). We demonstrate the expression of novel gene transcripts in the human endometrium. i.e. mucin-9, novel oestrogen-responsive gene transcripts, i.e. gelsolin and flotillin-1, and genes known to be expressed in human endometrium but not yet shown to be oestrogen responsive, i.e. connexin-37 and TFF1/pS2. Genes reported to be expressed during the implantation window and implicated in progesterone action, i.e. secretoglobin family 2A, member 2 (mammaglobin) and homeobox-containing proteins, were up-regulated in uncultured LATE-P-phase endometrium compared to M-phase endometrium. Some gene transcripts are regulated directly by 17beta-E2 alone, others are influenced by the in vivo environment as well. These observations emphasise that the regulation of endometrium maturation by oestrogen entails more then just stimulation of cell proliferation.