GSK-3β is essential for physiological electric field-directed Golgi polarization and optimal electrotaxis

• DOI: 10.1007/s00018-010-0608-z
• Publication Date: 2011-01-04T12:47:38Z
• Authors: Lin Cao; Jin Pu; Min Zhao
• Keywords: Cell polarity

Endogenous electrical fields (EFs) at corneal and skin wounds send a powerful signal that directs cell migration during wound healing. This therefore may serve as fundamental regulator directing polarization migration. Very little is known of the intracellular molecular mechanisms mediate EF-induced Here, we report Chinese hamster ovary (CHO) cells show robust directional in physiological EF (0.3–1 V/cm) both dissociated culture monolayer culture. An 0.6 V/cm completely abolished into higher strength (≥1 an overriding guidance cue for Application induced quick phosphorylation glycogen synthase kinase 3β (GSK-3β) which reached peak early 3 min EF. Inhibition protein C (PKC) significantly reduced directedness initially (in 1–2 h). GSK-3β GA inhibited migration, but later time (2–3 h EF). Those results suggest essential Golgi apparatus (GA) optimal electrotactic