Expression of a SOX1 overlapping transcript in neural differentiation and cancer models

• DOI: 10.1007/s00018-017-2580-3
• Publication Date: 2017-07-03T12:48:29Z
• Authors: Azaz Ahmad; Stephanie Strohbu...; Cristina Tufarelli; Virginie Sottile
• Keywords: 0301 basic medicine; 570; 571; SOX1; Messenger; Real-Time Polymerase Chain Reaction; Models, Biological; Cell Line; 03 medical and health sciences; Neural Stem Cells; Models; Cancer model; Animals; Humans; Neural Stem Cell; Expression pattern; RNA, Messenger; SOX1-OT; Stem cell; Animal; SOXB1 Transcription Factors; Chromosome Mapping; Cell Differentiation; Biological; Stem cell, LncRNA, SOX1-OT, expression pattern, cancer model, SOX1; LncRNA; 3. Good health; Genetic Loci; RNA; Long Noncoding; Original Article; RNA, Long Noncoding; Human

SOX1 is a member of the SOXB1 subgroup transcription factors involved in early embryogenesis, CNS development and maintenance neural stem cells. The structure regulation human locus has been less studied than that SOX2, another for which an overlapping transcript reported. Here we report harbours (SOX1-OT), describe expression, splicing variants detection SOX1-OT different cancer RT-PCR RACE experiments were performed to detect characterise neuroprogenitor cultures across cell lines. was found present complex including several unannotated exons, at least two potential start sites. be highly expressed differentiated cells time points differentiation, its expression correlated with gene expression. Concomitant further observed models. While function this unknown, regulatory role reported other lncRNAs strongly suggests possible regulating as previously SOX2. elucidation genetic context governing will contribute clarifying differentiation tumorigenesis.