MiR-17 and miR-19 cooperatively promote skeletal muscle cell differentiation

• DOI: 10.1007/s00018-019-03165-7
• Publication Date: 2019-06-18T16:04:46Z
• Authors: Delin Kong; Mei He; Lin Yang; Rongtao Zhou; Yun-Qin Yan; Yang Liang; Chun-Bo Teng
• Keywords: 0301 basic medicine; Cell Differentiation; Janus Kinase 1; Muscle Development; Myoblasts; Mice; MicroRNAs; 03 medical and health sciences; rhoC GTP-Binding Protein; Animals; Cyclin D2; Original Article; Cattle; Gene Regulatory Networks; Muscle, Skeletal; Cell Proliferation

Skeletal myogenesis is a highly coordinated process that involves cell proliferation, differentiation and fusion controlled by complex gene regulatory network. The microRNA cluster miR-17-92 has been shown to be related this process; however, the exact role of each member remains unclear. Here, we show miR-17 miR-20a could effectively promote both C2C12 myoblasts primary bovine satellite cells. In contrast, miR-18a might play negative in differentiation, while miR-19 miR-92a had little influence. Transcriptome target analyses revealed act on Ccnd2, Jak1 Rhoc genes are critical for proliferation and/or fusion. Notably, addition reverse lethal effect thus facilitate maturation myotubes. Furthermore, co-injecting lentiviral shRNAs into mouse tibialis anterior muscles, demonstrated wound healing abilities two miRNAs. Our findings indicate combination with miR-19, potent inducer skeletal muscle differentiation.