Experimental colonization with Blastocystis ST4 is associated with protective immune responses and modulation of gut microbiome in a DSS-induced colitis mouse model

• DOI: 10.1007/s00018-022-04271-9
• Publication Date: 2022-04-18T07:03:24Z
• Authors: Lei Deng; Lukasz Wojciech; Chin Wen Png; Eileen Yiling Koh; Thet Tun Aung; Dorinda Yan Qin Kioh; Eric Chun Yong Chan; Benoit Malleret; Yongliang Zhang; Guangneng Peng; Nicholas Robert J...; Kevin Shyong Wei Tan
• Keywords: Blastocystis

Blastocystis is a common gut protistan parasite in humans and animals worldwide, but its interrelationship with the host microbiota mucosal immune responses remains poorly understood. Different murine models of colonization were used to examine effect subtype (ST4) on microbial community adaptive system.Blastocystis ST4-colonized normal healthy mice Rag1-/- asymptomatically was able alter composition, mainly leading increases proportion Clostridia vadinBB60 group Lachnospiraceae NK4A136 group, respectively. ST4 promoted T helper 2 (Th2) response defined by interleukin (IL)-5 IL-13 cytokine production, regulatory (Treg) induction from colonic lamina propria mice. Additionally, we observed that can maintain stability bacterial composition induce Th2 Treg promote faster recovery experimentally induced colitis. Furthermore, fecal transplantation ST4-altered microbiome colitis reduced severity colitis, which associated increased production short-chain fat acids (SCFAs) anti-inflammatory IL-10.The data confirm our hypothesis beneficial commensal, effects mediated through modulating SCFAs different models.